UCsim2: Super-resolution microscopy for everyone with UC2 toolbox
- Abstract number
- 62
- Presentation Form
- Poster
- DOI
- 10.22443/rms.elmi2021.62
- Corresponding Email
- [email protected]
- Session
- Poster Session 2
- Authors
- Haoran Wang (1, 3), René Lachmann (1, 2), Barbora Marsikova (1, 2), Rainer Heintzmann (1, 3, 2), Benedict Diederich (1, 3)
- Affiliations
-
1. Leibniz Institute of Photonic Technology
2. Faculty of Physics and Astronomy, Friedrich-Schiller-University
3. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University
- Keywords
Modular Microscopy, Education, SIM, ISM, UC2
- Abstract text
Introduction:
This work demonstrates a compact multimodal setup based on a previously presented open-source optical toolbox UC2 [1] that combines two different super-resolution microscopy methods, image scanning microscopy (ISM) and structured illumination microscopy (SIM).
In this work, a new injection-moulded (IM) version of the UC2 toolbox is presented, which allows infinitely extending the baseplate with “puzzle”-like mapping mechanism in X/Y direction and “sandwich”-like stacking manner of multiple layers in Z direction. Injection-molding provides higher long-term stability and lower fabricating tolerances comparing to 3D printing. The IM version is downward-compatible with 3D-printed cubes which provides more possibility to assemble different-sized optical and electrical components into the setup.
The ISM and SIM can be realized in the UC2 framework at a low price and in a simplified setup benefited from consumer-grade laser video projector and open-source digital mirror device (DMD). The setup is used to imaging Alexa Flour 647(AF647)- and Silicon Rhodamine(SiR)-stained HeLa cells. The resolution of the system is determined as , and the total cost of the setup is down to ~2k€. The project information is open-accessed, devices as well as assembly details are available in the GitHub repository[2]. With the UC2 ecosystem, everyone can build their super-resolution microscopes in a low-cost way.
- References
[1] B. Diederich et al., “A versatile and customizable low-cost 3D-printed open standard for microscopic imaging,” Nat. Commun., vol. 11, no. 5979, pp. 1–9, 2020, doi: 10.1038/s41467-020-19447-9.
[2] B. Diederich, R. Lachmann, H. Wang, and B. Marsikova, “UC2 Github Hardware Repository,” 2019. https://github.com/bionanoimaging/UC2-GIT/tree/master/APPLICATIONS.
