Qualitative/Quantitative MINFLUX beyond the 1-nm resolution

Abstract number
80
Presentation Form
Poster
DOI
10.22443/rms.elmi2021.80
Corresponding Email
[email protected]
Session
Poster Session 2
Authors
Kirti Prakash (1)
Affiliations
1. National Physical Laboratory, London, UK
Keywords

MINFLUX, STED, SMLM, localisation precision, spatial resolution, super-resolution microscopy

Abstract text

Gwosch et al. (2020) and Balzarotti et al. (2017) purport MINFLUX as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1-3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focussing particularly on spatial resolution claims, atypical image rendering, visualisation enhancement, subjective filtering of localizations, detection vs labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions but also for microscope users, developers and core facility managers when deciding on an investment for the next 'state-of-the-art' instrument.


References

https://www.preprints.org/manuscript/202102.0173/v1