All-optical imaging of molecules in their nanoscale cellular context

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Super-Resolution Microscopy
Joerg Bewersdorf (1)
1. Yale School of Medicine, Yale University
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Super-resolution microscopy has become a powerful tool to study the nanoscale spatial distribution of proteins of interest in cells over the last years. Imaging any of these distributions in the context of other proteins or the general cellular context is, however, still challenging. I will present recent developments of our lab which tackle this challenge: A new fluorogenic DNA-PAINT probe enables fast, 3D whole-cell imaging without the need for optical sectioning, adding a versatile tool to the toolbox of single-molecule super-resolution probes [1]. Labeling proteins and other cellular components in bulk in our recent pan-Expansion Microscopy method provides ultrastructural context to the nanoscale organization of proteins, replacing complex correlative light/electron microscopy by an all-optical imaging approach [2]. 

Financial Interest Disclosure: J.B. has financial interest in Bruker Corp. and Hamamatsu Photonics and is co-founder of a startup company related to Expansion Microscopy.

[1] Chung, K.K.H. et al. “Fluorogenic probe for fast 3D whole-cell DNA-PAINT”. bioRxiv (2020). 

[2] M’Saad, O., Bewersdorf, J. Light microscopy of proteins in their ultrastructural context. Nat Commun 11, 3850 (2020).